Micro modification of the dilute blood clot lysis time for determining fibrinolytic activity.

Both methods gave excellent reproducibility and, particularly with the high sensitivity method, it was found that sample range could be varied by changing the sample volume aspirated and if necessary running it at 20°C, thus obviating full-scale deflection readings. The improvement in sensitivity with this method appears to result from the reaction with the phenol reagent, the initial biuret reaction appearing to go to completion very rapidly, neither time nor temperature within the conditions described having much effect. It is interesting to note that the reaction with 0-25 N phenol reagent appears to be independent of temperature, a phenomenon which is still being investigated.